Mouse brain atlas. Scroll down for more background on this atlas.

A Forum post Feb 8, 2019 · 2011-08: Status of Allen Brain Mouse atlas upgraded from "As Is" to "supported". A comprehensive three-dimensional digital atlas database of the C57BL/6J mouse brain was developed based on magnetic resonance microscopy images acquired on a 17. Full size image Benchmarking dataset ISH Data: Reference Atlas: AGEA: Brain Explorer: Related Studies Sleep; Mouse Strains; Documentation: Help Projection :: Allen Brain Atlas: Mouse Connectivity. This division is in a 16-year phase focused on multimodal characterization of brain cell types. Whole Transcriptome Atlas Profiling : >20,000 genes 5μm FFPE tissue section Area of Illumination (AOI) Profiling Strategy: 360 AOIs total; Geometric profiling in >10 functional areas of the brain Cell type-specific profiling of neurons across cortical layers and other functional areas; Morphology Markers: NeuN – Neurons; GFAP – Astrocytes Jun 26, 2020 · Abstract. A wide range of structural and functional experimental data mapped to the Allen mouse brain atlas are shared via the EBRAINS research infrastructure. "Search for mouse cells with electrophysiological properties similar to non-fast-spiking interneurons. Enhanced and Unified Mouse Brain Atlas at 10, 25, 50 and 100 micron resolutions; Smoothed version of the Kim et al. 5 mm 2), a mouse hemibrain (∼24. It provides neuroscientists with a unique insight into the cellular composition of the mouse brain. In 2021, we launched the Allen Institute for Neural Dynamics, a neuroscience division Mouse Brain Atlas. brain-map. In 2013, the reference atlas was updated to provide a deeper level of annotation. 1 to ∼76. A semi-automated procedure was used to conduct in situ hybridization and data About this atlas. This server is the gateway to The Portable Dictionary of the Mouse Genome (over 20,000 gene loci), The Mouse Brain Library, databases on mouse brain size Dec 13, 2023 · The whole mouse brain transcriptomic and spatial cell-type atlas establishes a benchmark reference atlas and a foundational resource for integrative investigations of cellular and circuit function Aug 16, 2023 · 19. 07/19/00 - Coronal DBA/2J atlas uploaded. Dec 13, 2023 · Here we report a comprehensive atlas of candidate cis -regulatory DNA elements (cCREs) in the adult mouse brain, generated by analysing chromatin accessibility in 2. Each data set is processed through an informatics analysis pipeline to obtain spatially mapped quantified expression information. l , Views of ISH experiment from the Allen Brain Atlas, showing spatial expression of Itih3 in the pallidum. 5 mouse brain atlas was registered to volumetric sample images. A single-cell transcriptomic atlas characterizes ageing tissues in the mouse. In an effort to expand our basic understanding of the neurobiology of the brain and its diseases, we performed a comprehensive molecular dissection of the main regions of the human, pig, and mouse brain using transcriptomics and antibody-based mapping. " Jul 28, 2021 · A comprehensive single-cell transcriptomic atlas of the mouse brain between gastrulation and birth identifies hundreds of cellular states and reveals the spatiotemporal organization of brain Feb 1, 2003 · The Allen Brain Atlas [7] contains detailed gene expression maps for ≈ 20, 000 genes in the C57BL/6J mouse [5]. Oct 15, 2021 · Aging mouse brain metabolome atlas. Correlation search for genes with similar spatial expression patterns. Aug 9, 2010 · Details on processing, imaging, calibration, and making brain data available online Mouse Brain Library Training Manual Training Manual - Detailed Protocol Mouse Brain Atlas Tutorial Information, images, and HTML for creating an atlas of your own MBL Slide Library Tutorial Step-by-step instructions for creating a slide database in FileMaker. org, launched in May 2005, is an interactive, multiresolution next-generation brain atlas that is based on over 140 million megapixels of sub-micron resolution, annotated, scanned images of serial sections of both primate and non-primate brains and that is integrated with a high-speed database for querying and retrieving data about brain 1. Dec 8, 2023 · The Allen Mouse Brain Atlas provides genome-wide in situ hybridization (ISH) image data for approximately 20,000 genes in adult mice. My software accounts for the subtle differences in page formatting and finds the Oct 16, 2020 · An LSFM-based mouse brain reference atlas containing an average anatomy template with corresponding brain region annotations was created. 28. Such atlases represent a key step towards understanding the functional genomics of the nervous system and provide a transcriptional baseline for 3d Viewer :: Allen Brain Atlas: Mouse Connectivity @description Oct 14, 2009 · The Allen Brain Atlas, a Web-based, genome-wide atlas of gene expression in the adult mouse brain, was an experiment on a massive scale. Dec 13, 2023 · Share: Six years and 32 million cells later, scientists have created the first full cellular map of a mammalian brain. Nov 28, 2018 · The atlas provides the densities and positions of all excitatory and inhibitory neurons, astrocytes, oligodendrocytes, and microglia in each of the 737 brain regions defined in the AMBA. The atlas comprises cellular resolution in situ hybridization images with comprehensive anatomic coverage that reveal where each gene is expressed in the mouse brain, as well as an integrated suite of powerful data search May 9, 2023 · The mouse brain atlas is a powerful tool that provides researchers with a detailed map of the mouse brain’s structure and function. Striatum. Jan 6, 2004 · This is a high resolution (2. Neuroinformatics 19, 433–446 (2020). Click on a button to view the corresponding section, or use the numbered guide in the frame below. Scroll down for more background on this atlas. Mouse and Rat Brain Atlas: An Interactive Online Tool. Genome-wide coverage. Robust decomposition of cell type mixtures in spatial transcriptomics. Using multimodal reference data, the entire brain parcellated directly in 3D, labeling every voxel with a brain Jan 29, 2024 · Register to attend a webinar on February 1, 2024, designed to share a new collection of papers reporting the first complete cell type atlas of a mammalian brain. Users can view and download the number, major types and 3D positions of all neurons and glia in all 737 areas of the mouse brain. Cable, D. Use the image below to select a section through the brain. Jan 5, 2024 · Given there are two structure ontologies for the mouse brain in the Allen Brain Atlas resources, we have created a way to overlay the Structure Ontology from Adult Mouse Atlas and the Developing Mouse Brain Atlas. The atlas provides the densities and positions of all excitatory and inhibitory neurons, astrocytes, oligodendrocytes, and Allen Mouse Brain Common Coordinate Framework (CCFv3, Wang et al, 2020) is a 3D reference space is an average brain at 10um voxel resolution created from serial two-photon tomography images of 1,675 young adult C57Bl6/J mice. Mar 15, 2018 · Using techniques to clear and expand mouse brains, scientists generate a compact brain atlas. Using the Brain Explorer 2 software, you can: View a fully interactive version of the ARA in 3-D. In 2021, we launched the Allen Institute for Neural Dynamics, a neuroscience division The Allen Mouse Brain Atlas is a three-dimensional atlas of gene expression throughout the adult mouse brain. The average mouse brain images have high signal-to-noise ratio and sharply defined boundaries of major white About the Allen Mouse Brain Atlas. Documentation: Allen Mouse Brain Atlas Document Description In Situ Hybridization Processes and procedures used to perform ISH Informatics Data Processing Design and implementation of the Informatics Data Pipel…. Tc1 Cerebellum : TC1 mouse cerebellar cortical sublayer lobules. The Brain Explorer 2 software is a desktop application for viewing the Allen Mouse Brain Atlas gene expression data in the framework of the Allen Reference Atlas (ARA). Key features: In situ hybridization data. National Institutes of Health Neuroscience Blueprint. For more information, please refer to the documentation . " Study: Mouse Cell Types. While in the “Developing Mouse Atlas, P56”, select “P56 Adult Mouse” atlas from the second drop-down menu and select The Allen Mouse Brain Atlas is a genome-wide, three-dimensional map of gene expression throughout the adult mouse brain. Earlier this year, many of the same techniques were used to assemble an initial atlas of the human A comprehensive and quantitative characterization of genes, cell types, and their spatial distribution across a whole mammalian brain is fundamental for understanding the function of the brain. In 2021, we launched the Allen Institute for Neural Dynamics, a neuroscience division Apr 2, 2014 · The Allen Mouse Brain Connectivity Atlas uses enhanced green fluorescent protein (EGFP)-expressing adeno-associated viral vectors to trace axonal projections from defined regions and cell types Oct 6, 2021 · While that ultimate goal remains to be achieved, researchers have steadily progressed with a smaller, but no less momentous, effort: an atlas of the mouse brain. Jan 23, 2020 · Many people find the digital reference atlases generated by the Allen Institute to be useful tools for educational, programmatically-accessible visualization and analysis. ) The Allen Brain Atlas API now offers access to our online data from across the suite of Allen Brain Atlas resources. 2 gm and a brain weight (fixed) of 477 mg. Anatomic reference atlas. Version 3 was released in 2015. Nuclei were identified brain-wide with a 3D blob detector algorithm, and the nuclei coordinates were used to map to specific region labels, allowing quantification of nuclei counts and density for each label. Of course, the mouse brain atlas is just one . The atlas is incorporated in the QuickNII The Allen Developing Mouse Brain Atlas includes a full-color, high-resolution anatomic reference atlas at seven timepoints accompanied by a systematic developmental taxonomy of mouse brain structures. Shrinkage during fixation and processing is 25% (linear). This is a high resoution (3. Isocortex. The mouse brain template was generated from 139 3D autofluorescence-scanned brain volumes by an iterative multi-resolution image registration algorithm (Fig. Advanced search and visualization tools. Using advanced technologies that profile individual cells, the teams Explore the interactive atlas viewer to access a comprehensive collection of brain anatomy and genetics data, with online tools for research and education. These images include T1-, T2*-, and T2-Weighted MR volumes (generated at the Duke Center for In-Vivo Microscopy), Nissl-stained optical histology (acquired at Drexel University), and a About this Atlas: The brain is that of a 51-day-old C57BL/6J male with a body weight of 20. 6-T superconducting magnet. Build the automated fluidic system; ROBOFISH. Browse adult spatial gene expression (440 or 168 genes measured, ~10,000 genes imputed). Among the advantages of electronic atlases, compared with the more conventional paper atlases, are that an electronic atlas can contain images of all sections, allowing the brain to be "re-sectioned" in any desired plane, can offer multiple levels of resolution and a range of colors, can present About this Atlas: The brain is that of a 294-day-old C57BL/6J male with a body weight of 34. It can be used to check the accuracy of the polygon extraction procedure (bitmap to curves). Dec 19, 2016 · Waxholm Space Atlas of the C57BL/6J Mouse Brain. About this Atlas: The brain is that of a 51-day-old C57BL/6J male with a body weight of 20. From the API, you can: Spatial atlas of the Adult Mouse Brain. Since its inception, the Allen Institute for Brain Science has assessed gene expression in the brain of mouse, human, and non-human primate in developing, adult, and aged individuals. [more] The Mouse Whole Brain Atlas is a high-resolution transcriptomic and spatial cell-type atlas across the entire mouse brain, integrating several whole-brain single-cell RNA-sequencing (scRNA-seq) datasets. Method. Our focus on neuroscience began with the launch of the Allen Institute for Brain Science in 2003, which led to the creation of the widely-used Allen Brain Atlases. The animal's celloidin case ID number is 165 and you can view the two slides ( a, b) used to make this atlas. Age. Mouse Brain Atlases. By using this atlas, researchers can gain new insights into complex behaviors, identify new neural circuits, and develop new treatments for neurodegenerative diseases. Oct 6, 2021 · Here we carry out a comprehensive assessment of the epigenomes of mouse brain cell types by applying single-nucleus DNA methylation sequencing 1, 2 to profile 103,982 nuclei (including 95,815 Mouse Cell Types: Similar to Non-Fast-Spiking Interneurons. FVB_NCrl: Brain MRI atlas of the wild-type FVB_NCrl mouse strain (used as the background strain for the rTg4510 which is a tauopathy model mice express a repressible form of human tau containing the P301L mutation that has been linked with familial frontotemporal dementia. The datasets contain a total of ~4 million cells passing rigorous quality-control (QC) criteria. Here, using snRNA-seq and Stereo-seq techniques, we generated a mouse brain atlas with spatial information for 29,655 genes as well as 308 cell clusters This atlas is still in the early stages of refinement. The Allen Reference Atlas is created by the Allen Institute for Brain Science. Principle Investigator: Nathaniel Heintz. Mar 6, 2020 · The brain is the most complex organ of the mammalian body, boasting a diverse physiology combined with intricate cellular organization. Mouse Brain Atlas: DBA/2J Horizontal. based on gene expression shifts. Brain Explorer® 3-D viewer. The Allen mouse brain atlas is a comprehensive digital resource that provides detailed information on the structure and function of the mouse brain. Perform image analysis with Feb 11, 2021 · The E18. It is published on the web (3D Coronal version) and in print: H. Oct 6, 2021 · Allen Mouse Brain Atlas. We aimed to generate a systematic classification of the adult mouse brain based purely on the unbiased identification of spatially defining features by employing whole-brain spatial transcriptomics. The atlas is separated into four key features: In Situ Hybridization (ISH) Data includes image data with cellular level resolution. 2 cm for different tissue sizes, subdivided into 50, 100, and 200 mm 2 chips for profiling sections from the mouse olfactory bulb (∼10. Sep 27, 2023 · a,b, Spatial plots of 20 sample slices coloured by CCF anatomical labels according to the Allen Institute 3D Mouse Brain Atlas 20 (a) and top-level molecularly defined tissue regions (b). " Mouse Phenome Databases & Mouse Colony Programs. Structure of the 3-vesicle brain as shown in diagrammatic representation, H&E-stained sagittal section and gross image showing the prosencephalon (PRO), mesencephalon (MS), rhombencephalon (RHO) and spinal cord (SC) in a E9. W. A, C, E. 2 mm 2) and whole mouse embryos (∼7. Including Nissl! 2011-08: 3D view now fits better in its frame. In 2021, we launched the Allen Institute for Neural Dynamics, a neuroscience division Sep 21, 2023 · Atlas images in A–C courtesy of mouse. The animal's celloidin case ID number is 232 and you can view one of the slides used to make this atlas. Population averaged T 2-weighted and Direction-encoded colormap (DEC) images of the mouse brain at several stages are shown in Figure 6 with segmentation of major gray and white matter structures. 5 µm/pixel) atlas in 8-bit color. Earlier this year, many of the same techniques were used to assemble an initial atlas of the human Brain Explorer® 2. 6µm/pixel) atlas in 8-bit color. For instance, we found substantial region-dependence in the magnitude and timing of C4b expression ( Figure 1 E), a complement component and major schizophrenia risk factor. 3 million individual brain Dec 9, 2021 · An optimized mouse brain atlas for automated mapping and quantification of neuronal activity using iDISCO+ and light sheet fluorescence microscopy. The Allen Mouse Brain Common Coordinate Framework is the backbone of spatially-focused workflows and tools that support spatial registration of new data to the atlas framework, semi-automatic analyses using the brain region hierarchy and delineations, and visualisation of extracted data in 3D. A Molecular Atlas of the Adult Mouse Brain. 2011-08: WHS09 and WHS10 templates show additional overlay 'rgb'. The atlas is dynamic, allowing comparison with previously reported numbers, addition of cell types, and improvement of estimates as new data is integrated. We constructed an average template brain at 10 μm voxel resolution by interpolating high resolution in-plane serial two-photon tomography images with 100 μm z-sampling from About this Atlas: The brain is that of a 294-day-old C57BL/6J male with a body weight of 34. org experiment ID 509463587, 603717226, and 510582887. But some people want to use a ‘classically printed’ atlas, on paper or in a PDF. 2 mg. Although various annotation volumes (AVs) for the mouse brain have been proposed, it is common in magnetic Dec 14, 2023 · "The atlas includes approximately 1 million cCREs and their chromatin accessibility across 1,482 distinct brain cell populations, adding over 446,000 cCREs to the most recent such annotation in the mouse genome," the authors reported, noting that the cCREs found in the mouse brain "are moderately conserved in the human brain. mouse reference atlas at 10, 25, 50 and 100 micron resolutions; Gubra's LSFM mouse brain atlas at 20 micron resolution; 3D version of the Allen mouse spinal cord atlas at 20 x 10 x 10 micron resolution Dec 13, 2023 · This study presented a single-cell DNA methylation and 3D multi-omic atlas of the entire mouse brain. The animal's celloidin case ID number is 170 and you can view one of the slides used to make this atlas. The in vivo grid compensates for this Nov 27, 2018 · Here we provide a first 3D cell atlas for the whole mouse brain, showing cell positions constructed algorithmically from whole brain Nissl and gene expression stains, and compared against values from the literature. Oct 11, 2018 · Recent efforts seek to create a comprehensive cell atlas of the human body [1, 2]. The Blue Brain Cell Atlas is the first digital 3D cell atlas of the whole mouse brain. " About this Atlas: The brain is that of a 76-day-old DBA/2J female with a body weight of 20. In a set of 10 papers in Nature today, a network of researchers unveiled an atlas cataloging the location and type of every cell in the adult mouse brain. A genome-wide, high-resolution atlas of gene expression throughout the adult mouse brain. Microscopic resolution down to the cellular level. Download. The development of the atlas faced a combination of great Nov 7, 2019 · For the mouse, the most widely used animal model to understand the mammalian brain, a variety of printed and/or digital atlases exist with varying levels of segmentations in 2D or 3D images Brain atlases are as essential for the research neuroscientist as are maps for the geographer. This is the online help for the ALLEN Mouse Brain Atlas web application. The advent of single-cell and single-nucleus mRNA sequencing (RNAseq) in droplet format [3,4,5] now enables large-scale sampling of cells from any tissue, and clustering of these large-scale datasets enables cell type and subtype classification [3, 6,7,8]. 1a). Version 2 was released in 2011. GENSAT Brain Atlas of gene expression in EGFP Transgenic Mice. org. To find gene expression data, search can be performed using Gene Search, Differential Search, Fine ISH Data: Reference Atlas: AGEA: Brain Explorer: Related Studies Sleep; Mouse Strains; Documentation: Help Mar 4, 2024 · In 2021, researchers supported by the BRAIN Initiative—including teams at Salk—unveiled the first draft of the mouse brain atlas, which pioneered new tools to characterize neurons and applied those tools to small sections of the mouse brain. Shrinkage during fixation and processing is 21% (linear). Aug 31, 2005 · Mouse Brain Library Training Manual Training Manual - Detailed Protocol Mouse Brain Atlas Tutorial Information, images, and HTML for creating an atlas of your own MBL Slide Library Tutorial Step-by-step instructions for creating a slide database in FileMaker. The in vivo grid compensates for this shrinkage. Here you can download image volumes representing the canonical Waxholm Space (WHS) adult C57BL/6J mouse brain. work, in stereotactic coordinates. 95 gm and a brain weight (fixed) of 476. By utilizing methylome-based clustering and cross-modality integration with additional BICCN Our focus on neuroscience began with the launch of the Allen Institute for Brain Science in 2003, which led to the creation of the widely-used Allen Brain Atlases. May 14, 2020 · Successful integration of these data requires a standard 3D reference atlas. Gene Expression. Filter the results by the Cre line and the availability of morphology, data models, or a 3-D reconstruction. edu ***** Code may be re-used for non-commercial use. Histological images in D from connectivity. Each dot May 4, 2022 · So far, we have used Stereo-seq chips of up to an effective area of 13. I’ve created online tools for the Rat Brain Atlas and Mouse Brain Atlas based on the Paxinos et al. Post-processing of the template involved In recent years, the combination of whole-brain immunolabelling, light sheet fluorescence microscopy (LSFM) and subsequent registration of data with a common reference atlas, has enabled 3D visualization and quantification of fluorescent markers or tracers in the adult mouse brain. Here, we present the Allen Mouse Brain Common Coordinate Framework (CCFv3) as such a resource. The markers above are spaced 1 mm apart and correspond to levels of the Atlas. Available from: atlas. Co-Investigator: High Resolution Mouse Brain Atlas. Key features: In situ hybridization image data with cellular-level resolution. Projection BDA/AAV Comparison Transgenic Characterization Reference Data Brain Explorer Documentation Help. The Allen Mouse Brain Atlas includes a full-color, high-resolution anatomic reference atlas accompanied by a systematic, hierarchically organized taxonomy of mouse brain structures. Dec 13, 2023 · In 2021, researchers supported by the BRAIN Initiative—including teams at Salk—unveiled the first draft of the mouse brain atlas, which pioneered new tools to characterize neurons and applied those tools to small sections of the mouse brain. Neurogenetics at the University of Tennessee, Memphis. 2 cm × 13. Atlas was created based on the original MRM NeAt mouse brain atlas (template images reoriented and bias-corrected, left/right structure label seperated, and 4th ventricle manual segmentation added). harvard. By using both manual tracing and an atlas-based semi-automatic segmentation approach, T2-weighted magnetic res … Alternative Background Images; Average Template; Atlas; Mouse Atlases; Adult, 3D Coronal; P56, Coronal; P56, Sagittal; Developing Mouse Atlases; P56, Sagittal Mouse Brain Atlas. 8/16/1999 - On-line brain atlas project started. Read the EEL preprint . View gene expression data in 3-D at 200 μm 3 resolution. Project Officer: Laura Mamounas. The whole-brain molecular atlas further supports the identification of the spatial origin of single neurons using their gene expression profile, and forms the foundation to define a minimal gene set - a brain palette - that is sufficient to spatially annotate the adult brain. List of atlases. Download the data. 07/16/01 - Horizontal DBA/2J Atlas. Comprehensive anatomic coverage. An atlas of cell types from the Linnarsson Lab. This mouse cerebellar atlas can be used for mouse cerebellar morphometry. The Allen Developing Mouse Brain Atlas includes a full-color, high-resolution anatomic reference atlas at seven timepoints accompanied by a systematic developmental taxonomy of mouse brain structures. Supported by: National Institute of Neurological Disorders and Stroke, contract # NO1-NS-7-2370. BrainMaps. Although the original Allen Mouse Brain Atlas API is still available, the latest 3-D grid-level expression summaries, reference atlas, and services for the Mouse Brain Atlas are available via the Allen Brain Atlas API. Dong, The Allen Reference Atlas, (Book + CD-ROM): A Digital Color Brain Atlas of the C57BL/6J Male Mouse, Wiley, 2008. The atlas enables brain-wide assessments of the number, distribution, and activities of labeled cells. Full size image Our focus on neuroscience began with the launch of the Allen Institute for Brain Science in 2003, which led to the creation of the widely-used Allen Brain Atlases. next generation brain maps and brain atlases. et al. Explore the structure and function of the mouse brain with the Interactive Atlas Viewer, a tool that integrates multiple data types and atlases from the Allen Brain Atlas project. Mouse Brain MRI atlas (both in-vivo and ex-vivo) (repository relocated from the original webpage). Design probes with oligopy. Browse interactively using FISHscale: Try the protocol. Brain maps are essential for integrating information and interpreting the structure-function relationship of circuits and behavior. Created by: Edmund Cape Last updated: Dec 16th 1999 By: Edmund Cape email: Edmund_Cape@hms. 1 mm 2), respectively (Figure S1A). This tutorial gives detailed instructions for creating your own mouse brain atlas. Protocol and Code. 1 mg. In collaboration with the UC Davis Mouse Biology Program, we have studied groups of 8 male and 8 female wildtype mice at AD (3 weeks), early adulthood (EA, 16 Jan 1, 2011 · Population-Averaged Mouse Brain Atlas. Dec 13, 2023 · The Allen Reference Atlas: A Digital Color Brain Atlas of the C57Bl/6J Male Mouse (Wiley, 2008). The integrated transcriptomic taxonomy Projection :: Allen Brain Atlas: Mouse Connectivity. M. Mar 18, 2021 · A brain atlas is necessary for analyzing structure and function in neuroimaging research. Each of its 72 million dots represents a single cell. Shrinkage during fixation and processing is 24% (linear). 7 gm and a brain weight (fixed) of 401. ISH Data: Reference Atlas: AGEA: Brain Explorer: Related Studies Sleep; Mouse Strains; Documentation: Help The Allen Mouse Brain Atlas is a genome-wide, high-resolution atlas of gene expression throughout the adult mouse brain. 0 mouse embryo. A collection of papers published in Nature last month revealed a comprehensive and multi-modal profile of cells across the entire mouse brain. Coronal C57BL/6J atlas completed. Are these available? This post explains how to produce printable versions of the human and mouse brain reference atlases. Embryonic transformation of the mouse brain from a 3- to a 5-vesicle structure. 07/26/00 - Mouse Brain Atlas Tutorial and related files added. In a special issue of Nature, publishing online October 7, 2021, researchers at the University of California San Diego, with colleagues across the country, describe their progress in Dec 8, 2023 · Allen Mouse Brain Atlas. Brain atlases. “This is an important step forward in analyzing brain structure and connectivity. All the sections have been extracted from the original PDF documents. er qm bp hf os vo xv nl wx ac

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